Methods

• Xenograft mouse model system
• Detection of cellular senescence marker, apoptosis, stem cell character - stemness
• Production of spheroids
• Ex vivo analysis of human prostate cancer tumor samples
• Analysis of circulating tumor cells of prostate cancer patients
• RNA-seq - transcriptome analyzes
• ChIP-qPCR; ChIP-seq

Methods of DNA-protein-protein interaction:

• Chromatin Immunoprecipitation (ChIP)
• Co-immunoprecipitation
• EMSA
• Mammalian cell cultures

Hormonal regulation of gene expression:

• qRT-PCR
• DNA transfection in cell cultures
• Reporter assays